special stain
Martius scarlet blue (MSB)
Function: stain fibrin
Principle: the main feature of MSB is the use of a small molecule yellow dye, together with phosphotungstic acid to selectively stain red cells. Phosphotungstic acid blocks the staining of muscle, collagen and most connective tissue fibres. Muscle and fibrin are stained with medium size molecule red dye, while staining of collagen is prevented by phosphotungstic acid. Larger blue dye will then stained the collagen and fibrin.
Control: skeletal or cardiac muscle, soft cartilage, fibrinous appendix
Chemical required: 1% acetic acid
2%phosphotungstic acid
martius blue
martius red
martius yellow
weigert iron haematoxylin
Reagent preparation: martius scarlet blue working solution
martius yellow 1 part
martius blue 2 parts
martius red 3 parts
Procedure:
1) dewax, section to water
2) leave slide overnight in zenker’s solution
3) run in water until colourless
4) stain with weigert iron haematoxylin for 10 minutes
5) blue in water
6) stain in martius scarlet blue working solution for 10 mins
7) wash with acetic acid
8) dehydrate, clear, mount
Result: nuclei: blue/black
Erythrocyes: yellow
Fibrin: red
Connective tissue: blue
Wing Fat
TG01
Function: stain fibrin
Principle: the main feature of MSB is the use of a small molecule yellow dye, together with phosphotungstic acid to selectively stain red cells. Phosphotungstic acid blocks the staining of muscle, collagen and most connective tissue fibres. Muscle and fibrin are stained with medium size molecule red dye, while staining of collagen is prevented by phosphotungstic acid. Larger blue dye will then stained the collagen and fibrin.
Control: skeletal or cardiac muscle, soft cartilage, fibrinous appendix
Chemical required: 1% acetic acid
2%phosphotungstic acid
martius blue
martius red
martius yellow
weigert iron haematoxylin
Reagent preparation: martius scarlet blue working solution
martius yellow 1 part
martius blue 2 parts
martius red 3 parts
Procedure:
1) dewax, section to water
2) leave slide overnight in zenker’s solution
3) run in water until colourless
4) stain with weigert iron haematoxylin for 10 minutes
5) blue in water
6) stain in martius scarlet blue working solution for 10 mins
7) wash with acetic acid
8) dehydrate, clear, mount
Result: nuclei: blue/black
Erythrocyes: yellow
Fibrin: red
Connective tissue: blue
Wing Fat
TG01
posted by BloodBank.MedMic.Haematology @ 9:31 PM
7 Comments:
At November 5, 2007 at 2:23 PM ,
first6weeks said...
hihi wing fatt,
in what circumstances to use special stains?
At November 5, 2007 at 2:23 PM ,
first6weeks said...
hihi Wing Fatt
in what circumstances to use special stains?
Juexui
tg02
At November 6, 2007 at 9:40 PM ,
we are the XiaoBianTai-7! said...
hey wing fatt,
can i know whats the part in the components u mentioned for the martius scarlet blue solution?
Ai Tee
0503160D
At November 7, 2007 at 4:35 PM ,
first6weeks said...
HELLO
What are the discrepancy during the interpretattion of result?
Ching Wei
At November 10, 2007 at 4:53 PM ,
Star team said...
hey wing fatt,
are there other dye use to stain fibrin?
you said that: Phosphotungstic acid blocks the staining of muscle, collagen and most connective tissue fibres. Muscle and fibrin are stained with medium size molecule red dye. So is the muscle tissue stained? haha.
phuiyuen, tg 02
At November 26, 2007 at 1:16 PM ,
ALsubs said...
hello
what tests are used for this stains and what does the stain look out for?
Dorene TG02
At November 27, 2007 at 8:27 PM ,
BloodBank.MedMic.Haematology said...
To Jue xui
special stains are used to demonstrate tissue structures that are normally not demonstrated by H&E.
To Ai Tee:
martius scarlet blue solution is the combination of 1 part of martius yellow, 2 parts of martius blue and 3 parts of martius red. It is a working solution used in the staining procedure.
To ching wei:
So far there are no known discrepancy during interpretation of results in our lab.
To Phui Yuen:
yes, such as the masson's trichrome.
phosphotungstic acid blocks the YELLOW DYE (sorry if i didnt mention that!)form staining the muscle but muscles will be stained red upon treatment with the medium size molecule red dye.
To doreen:
It is used to demostrate fibrins and connective tissues. Usually used in tissue sample such as skeletal or cardiac muscle, soft cartilage or fibrinout appendix.
Wing Fat
Tg01
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